Contents:
Sabrina added it Jul 16, Astrid Freese added it Aug 26, Helena marked it as to-read Mar 27, Criwa marked it as to-read Sep 08, Sabrina added it Nov 17, Yasminchen added it Jan 10, Nadine added it Apr 27, Giu marked it as to-read Jun 19, There are no discussion topics on this book yet.
Books by Petra Frank-Herrmann. No trivia or quizzes yet.
Odeblad E The functional structure of human cervical mucus. Acta Obstet Gynecol Scand Elsevier, Amsterdam, pp Am J Obstet Gynecol Hartman CG Science and the safe period. Williams and Wilkins, Baltimore, MD Hillier S Current concepts of the roles of follicle stimulating hormone and luteinizing hormone in folliculogenesis.
Skip to content admin December 25, Psychology of Wounds and Wound Care in Clinical Practice by Dominic Upton, Penney Upton This booklet addresses the mental effects of wounds and the mental elements of wound care, development on modern study facts and proposing sensible scientific instructions. The mouse was sacrificed by cervical dislocation immediately after the second oocyte retrieval.
After routine paraffin embedding, the entire sample was cut in serial sections and stained with hematoxylin and eosin for histological examination. Classification of follicles based on their morphological appearance was as follows:. All developmental stages of follicles could be identified during investigation of histological serial sections; primordial, primary, preantral as well as antral follicles with intact morphology [ Fig.
In addition, some large atretic follicles were present in the ovarian tissue part as well as multinucleated oocytes [ Fig.
Furthermore, several clusters with dormant primordial follicles could be identified [ Fig. In terms of safety, previous studies show contrasting results: Hence, more studies would be necessary to clarify if ovarian tissue re-transplantation after cancer treatment is safe or not; for each malignancy entity separately. Using the xenografting method to restore fertility would be a potential capability to circumvent the risk of reintroducing cancer through reimplantation of tissue which contains malignant cells; in short, xenografting potentially contaminated tissue with the objective of receiving mature oocytes for in vitro fertilization and later embryo replacement.
Linked Data More info about Linked Data. No trivia or quizzes yet. After routine paraffin embedding, the entire sample was cut in serial sections and stained with hematoxylin and eosin for histological examination. Repetitive oocyte retrieval cycles can be achieved with non-stimulated xenografts of prepubertal ovarian tissue; this highlights the potential of prepubertal ovarian tissue. Home About Help Search.
From the methodical point of view, it would be a convenient technique, as cancer cells are not able to pass the Zona pellucida. Nevertheless, there are ethical and potential safety issues, which are not answered yet and have to be addressed in further research [ 20 ]. A further approach for safety reasons is in vitro maturation IVM of oocytes; different options are imaginable and already subject of intensive research: For instance, in vitro culturing of whole human ovarian tissue or isolated follicles alone with the aim to activate follicle development and receive mature oocytes for cryopreservation or subsequent in vitro fertilization; an additional IVM period might be necessary in case collected oocytes are not completely mature.
Another possibility is oocyte aspiration from removed ovarian tissue prior to cryopreservation, combined with IVM culturing of oocytes either before freezing or after warming; this has already been done with prepubertal tissue [ 29 ]. Another, very promising alternative to autografting of frozen-thawed ovarian tissue is transplanting an artificial ovary.
During this method, isolated follicles from ovarian tissue attached to or encapsulated in an artificial matrix are provided for transplantation instead of whole tissue; the main idea is to get rid of potential malignant cells during this processing and thus avoid reintroducing cancer. Altogether, there are many different basics approaches, most of them reviewed in [ 30 ] and a recent report by Laronda et al.
Our histological evaluation showed almost all developmental stages of follicles primordial, primary, preantral as well as antral follicles and several primordial follicle clusters. This is in accordance with the findings by Luyckx et al.
Interestingly, we could observe a conformable follicular development without any exogenous hormone stimulation. To date, this study by Luyckx et al. The described repetitive retrieval of a maturing as well as a mature oocyte from the same xenograft of prepubertal human ovarian tissue without exogenous hormone stimulation is, to our best knowledge, the first report of that kind. Retrieving mature oocytes from unstimulated xenografts corresponds with similar results from Lotz et al.
In summary, Lotz et al. One of the metaphase II oocytes originated in the control without exogenous stimulation and in absence of hCG supply [ 32 ].